Genetics Healthcare Camden Group

A Simple Screening Method for Superior RNA–DNA Hybrid Nanoshapes

The design of hybrid nucleic acid nanomaterials capitalizes on the partitioning of architectural and sensible roles between structurally numerous RNA modules and chemically robust DNA components. Selecting optimum combos of RNA and DNA developing blocks is the vital factor to preparing safe polygonal RNA-DNA hybrid nanoshapes. Proper right here, we outline a simple screening method by gel electrophoresis beneath native folding circumstances to find out combos of RNA and DNA modules that self-assemble to robust polygonal hybrid nanoshapes. As a proof of thought, we outline the preparation of RNA-DNA hybrid nanoshapes containing a set of varied RNA architectural joints, along with inside loop motifs and three-way junction (3WJ) folds.

For each hybrid nanoshape, we reveal the selection course of used to find out optimum DNA modules from a library of DNA connectors. The simple screening method outlined proper right here presents a fundamental robust methodology to find out and put collectively RNA-DNA hybrid nanoshapes from numerous libraries of discrete nucleic acid developing blocks. End resection in homologous recombination (HR) and HR-mediated restore of DNA double-strand breaks (DSBs) removes quite a lot of kilobases from 5′ strands of DSBs, nonetheless 3′ strands are exempted from degradation.

The mechanism by which the three’ overhangs are protected has not been determined. Proper right here, we established that the security of three’ overhangs is achieved by the use of the transient formation of RNA-DNA hybrids. The DNA strand throughout the hybrids is the three’ ssDNA overhang, whereas the RNA strand is newly synthesized. RNA polymerase III (RNAPIII) is accountable for synthesizing the RNA strand. Furthermore, RNAPIII is actively recruited to DSBs by the MRN superior. CtIP and MRN nuclease train is required for initiating the RNAPIII-mediated RNA synthesis at DSBs. A lowered stage of RNAPIII suppressed HR, and genetic loss > 30 bp elevated at DSBs. Thus, RNAPIII is a obligatory HR problem, and the RNA-DNA hybrid is a obligatory restore intermediate for shielding the three’ overhangs in DSB restore.

Sequence-independent RNA sensing and DNA specializing in by a break up space CRISPR-Cas12a gRNA change

CRISPR utilized sciences an increasing number of require spatiotemporal and dosage administration of nuclease train. One promising method contains linking nuclease train to a cell’s transcriptional state by engineering data RNAs (gRNAs) to carry out solely after complexing with a ‘set off’ RNA. Nonetheless, regular gRNA change designs do not allow neutral selection of set off and knowledge sequences, limiting gRNA change software program. Proper right here, we reveal the modular design of Cas12a gRNA switches that decouples selection of those sequences. The 5′ end of the Cas12a gRNA is fused to 2 distinct and non-overlapping domains: one base pairs with the gRNA repeat, blocking formation of a hairpin required for Cas12a recognition; the alternative hybridizes to the RNA set off, stimulating refolding of the gRNA repeat and subsequent gRNA-dependent Cas12a train.

Using a cell-free transcription-translation system and Escherichia coli, we current that designed gRNA switches can reply to fully completely different triggers and aim fully completely different DNA sequences. Modulating the scale and composition of the sensory space altered gRNA change effectivity. Lastly, gRNA switches might very effectively be designed to sense endogenous RNAs expressed solely beneath specific progress circumstances, rendering Cas12a specializing in train relying on cell metabolism and stress. Our design framework thus extra permits tethering of CRISPR actions to cell states.

Total RNA from Diabetic Disease: Stomach
R1236248Dia-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Stomach
R1235248-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Stomach
R1434248-50	Biochain	50 ug	EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach
R1234248-50	Biochain	50 ug	EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Monkey (Rhesus) Normal Tissue: Stomach
R1534248-50	Biochain	50 ug	EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Monkey (Cynomolgus) Normal Tissue: Stomach
R1534248-Cy	Biochain	50 ug	EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Congenital heart disease: Heart
R1236122Hd-3	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach: Cardia
R1234250-10	Biochain	10 ug	EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach: Corpus
R1234251-10	Biochain	10 ug	EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach: Fundus
R1234252-10	Biochain	10 ug	EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach: Pylorus
R1234253-10	Biochain	10 ug	EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Mouse Normal Tissue: Stomach
P1334248	Biochain	1 mg	EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Adult Normal Tissue 5 Donor Pool: Stomach
R1234248-P	Biochain	50 ug	EUR 328
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL	Cell Biolabs	96 assays	EUR 572

Matching Pair - Total RNA from Human Primary and Metastatic Tumor Tissue: Stomach
R8235248-PM-10	Biochain	2x10 ug	EUR 650
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Matching Pair - Total RNA from Human Primary Tumor and Normal Tissue: Stomach
R8235248-PP-10	Biochain	2x10 ug	EUR 439
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Navy RNA Lysis Kit 50
NAVYE1-RNA	Next Advance	50pack	EUR 198
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250
NAVYE5-RNA	Next Advance	250pack	EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Green RNA Lysis Kit 50
GREENE1-RNA	Next Advance	50pack	EUR 180
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250
GREENE5-RNA	Next Advance	250pack	EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Pink RNA Lysis Kit 50
PINKE1-RNA	Next Advance	50pack	EUR 178
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250
PINKE5-RNA	Next Advance	250pack	EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50
YELLOWE1-RNA	Next Advance	50pack	EUR 192
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250
YELLOWE5-RNA	Next Advance	250pack	EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50
REDE1-RNA	Next Advance	50pack	EUR 192
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250
REDE5-RNA	Next Advance	250pack	EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E Navy RNA Lysis Kit 100
NAVY5E100-RNA	Next Advance	100pack	EUR 453
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20
NAVY5E20-RNA	Next Advance	20pack	EUR 153
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50
NAVYR1-RNA	Next Advance	50pack	EUR 202
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250
NAVYR5-RNA	Next Advance	250pack	EUR 666
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E GREEN RNA Lysis Kit 100
GREEN5E100-RNA	Next Advance	100pack	EUR 386
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20
GREEN5E20-RNA	Next Advance	20pack	EUR 138
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50
GREENR1-RNA	Next Advance	50pack	EUR 184
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250
GREENR5-RNA	Next Advance	250pack	EUR 587
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E PINK RNA Lysis Kit 100
PINK5E100-RNA	Next Advance	100pack	EUR 380
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20
PINK5E20-RNA	Next Advance	20pack	EUR 136
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50
PINKR1-RNA	Next Advance	50pack	EUR 181
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250
PINKR5-RNA	Next Advance	250pack	EUR 576
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50
YELLOWR1-RNA	Next Advance	50pack	EUR 199
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250
YELLOWR5-RNA	Next Advance	250pack	EUR 656
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100
RED5E100-RNA	Next Advance	100pack	EUR 429
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20
RED5E20-RNA	Next Advance	20pack	EUR 148
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50
REDR1-RNA	Next Advance	50pack	EUR 195
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250
REDR5-RNA	Next Advance	250pack	EUR 638
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Total Protein from Human Tumor Tissue: Stomach
P1235248	Biochain	1 mg	EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Diabetic Tissue: Stomach
P1236248Dia	Biochain	1 mg	EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

RNA isolater Total RNA Extraction Reagent
R401-01	Vazyme	100 ml	EUR 133

Total RNA Isolation Kit
P-9100	EpiGentek	50 samples	EUR 347.25
Description: The best epigenetics products

Total RNA Extraction Kit
K2014005	Biochain	1 kit	EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Paraffin Tissue Section - Human Stomach Tumor: Adenocarcinoma, Well Differentiated
T2235248-3	Biochain	5 slides	EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total Protein from Emphysema: Lung
P1236152Ld-3	Biochain	1 mg	EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL-5	Cell Biolabs	5 x 96 assays	EUR 2283

Total Protein from Human Adult Normal Tissue: Stomach
P1234248	Biochain	1 mg	EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Mouse Normal Tissue: Adipose
R1334003-50	Biochain	50 ug	EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Bladder
R1334010-50	Biochain	50 ug	EUR 303
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Brain
R1334035-50	Biochain	50 ug	EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Colon
R1334090-50	Biochain	50 ug	EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Heart
R1334122-50	Biochain	50 ug	EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Kidney
R1334142-50	Biochain	50 ug	EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Liver
R1334149-50	Biochain	50 ug	EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Lung
R1334152-50	Biochain	50 ug	EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Pancreas
R1334188-50	Biochain	50 ug	EUR 341
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Placenta
R1334200-50	Biochain	50 ug	EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Rectum
R1334206-50	Biochain	50 ug	EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Spleen
R1334246-50	Biochain	50 ug	EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Testis
R1334260-50	Biochain	50 ug	EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA Isolation Kit (Tissue)
P-9102	EpiGentek	50 samples	EUR 347.25
Description: reagents widely cited

Total RNA Isolation Kit (Plant)
P-9104	EpiGentek	50 samples	EUR 347.25
Description: kits suitable for this type of research

Total RNA Isolation Kit II
FATRS--001	Favorgen	100 preps	EUR 308

Total RNA Isolation Kit II
FATRS--050	Favorgen	50 preps	EUR 216

Total RNA ExtractionMini KitAnimal Tissues
FYG301-50P	Yeastern Biotech	50 Preps	Ask for price

Total RNA ExtractionMini KitAnimal Tissues
FYG302-100P	Yeastern Biotech	100 Preps	Ask for price

Total RNA ExtractionMini KitAnimal Tissues
FYG302-300P	Yeastern Biotech	300 Preps	Ask for price

Total RNA ExtractionMini KitPlant Tissues
FYG303-100P	Yeastern Biotech	100 Preps	Ask for price

Total RNA ExtractionMini KitPlant Tissues
FYG303-300P	Yeastern Biotech	300 Preps	Ask for price

Total RNA ExtractionMini KitPlant Tissues
FYG303-50P	Yeastern Biotech	50 Preps	Ask for price

AnaPrep Total RNA Extraction Kit
Z1322015	Biochain	1 kit (48 extractions) Including all required plastic disposables	EUR 308
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.

Total RNA from Lupus: Colon
R1236090Lup-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart
R1236122Hd-2	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Kidney
R1236142Lup-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Liver
R1236149Lup-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Lung
R1236152Lup-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Pancreas
R1236188Lup-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Spleen
R1236246Lup-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Uterus
R1236274Lup-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Congenital heart disease: Heart
P1236122Hd-3	Biochain	1 mg	EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Cardia
P1234250	Biochain	1 mg	EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Corpus
P1234251	Biochain	1 mg	EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Fundus
P1234252	Biochain	1 mg	EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Pylorus
P1234253	Biochain	1 mg	EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Mouse Normal Tissue: Whole Eye
R1334108-50	Biochain	50 ug	EUR 296
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Skeletal Muscle
R1334171-50	Biochain	50 ug	EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Small Intestine
R1334226-50	Biochain	50 ug	EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells
2-82088	CHI Scientific	1 Kit	Ask for price

Plant Total RNA Mini Kit (50prep)
FAPRK-001	Favorgen	50 preps	EUR 165

Plant Total RNA Mini Kit (100prep)
FAPRK-001-1	Favorgen	100 preps	EUR 216

Plant Total RNA Mini Kit (300prep)
FAPRK-001-2	Favorgen	300 preps	EUR 404

Plant Total RNA Maxi Kit (10prep)
FAPRK-002	Favorgen	10 preps	EUR 201

Plant Total RNA Maxi Kit (24prep)
FAPRK-002-1	Favorgen	24 preps	EUR 317

Tissue Total RNA Mini Kit (50prep)
FATRK-001	Favorgen	50 preps	EUR 165

Tissue Total RNA Mini Kit (100prep)
FATRK-001-1	Favorgen	100 preps	EUR 216

Tissue Total RNA Mini Kit (300prep)
FATRK-001-2	Favorgen	300 preps	EUR 404

Tissue Total RNA Maxi Kit (10prep)
FATRK-003	Favorgen	10 preps	EUR 201

Tissue Total RNA Maxi Kit (24prep)
FATRK-003-1	Favorgen	24 preps	EUR 317

Total RNA ExtractionMini KitBlood/Cultured Cell
FYG301-300P	Yeastern Biotech	300 Preps	Ask for price

Rapid Animal Total RNA Extraction Kit
AT4181	Bio Basic	50Preps	EUR 88.06

Rapid Animal Total RNA Extraction Kit
AT4182	Bio Basic	250preps	EUR 202.25

Zenoquick Whole Blood Total RNA Kit
Z5010-050	GenDepot	50 preps	EUR 482

Zenoquick Whole Blood Total RNA Kit
Z5010-100	GenDepot	2x50 preps	EUR 806

Broad Range Total RNA Isolation Kit
K1341050	Biochain	1 kit	EUR 266
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Alzheimer's Disease: Brain
R1236035Alz-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Pons
R1236071Alz-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Colon
R1236090Dia-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Colon
R1236090Lcs-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Esophagus
R1236106Dia-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Interventricular Septum
R1236130Hd-2	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Kidney
R1236142Lcs-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Liver
R1236149Lcs-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Lung
R1236152Lcs-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Diaphragm
R1236169Lcs-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Pancreas
R1236188Dia-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Pancreas
R1236188Lcs-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Rectum
R1236206Lcs-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Small Intestine
R1236226Lup-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Spleen
R1236246Lcs-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Stomach Lysate
1415	ProSci	0.1 mg	EUR 191
Description: Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate
1475	ProSci	0.1 mg	EUR 191
Description: Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate
1319	ProSci	0.1 mg	EUR 191
Description: Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

96-well Total RNA Kit (1 plates)
FATRE-96001	Favorgen	1 prep	EUR 194

96-well Total RNA Kit (2 plates)
FATRE-96002	Favorgen	2 preps	EUR 281

96-well Total RNA Kit (4 plates)
FATRE-96004	Favorgen	4 preps	EUR 448

Total RNA Plus Mini Kit (50 prep)
FATRK-P-050	Favorgen	50 preps	EUR 179

Total RNA Plus Mini Kit (100 prep)
FATRK-P-100	Favorgen	100 preps	EUR 242

Total RNA from Human Tumor Tissue: Adrenal
R1235004-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bladder
R1235010-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bone
R1235023-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Breast
R1235086-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Colon
R1235090-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Esophagus
R1235106-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Kidney
R1235142-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Liver
R1235149-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Lung
R1235152-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Nose
R1235178-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Ovary
R1235183-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Parotid
R1235190-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Rectum
R1235206-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Melanoma
R1235218A-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Synoviosarcoma
R1235257-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thymus
R1235264-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thyroid
R1235265-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Tonsil
R1235268-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Uterus
R1235274-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Corpus Callosum
R1236045Alz-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Frontal Lobe
R1236051Alz-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Occipital Lobe
R1236062Alz-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Parietal Lobe
R1236066Alz-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Postcentral Gyrus
R1236072Alz-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Precentral Gyrus
R1236073Alz-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Temporal Lobe
R1236078Alz-10	Biochain	10 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Atrium, left
R1236126Hd-2	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Atrium, right
R1236127Hd-2	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Ventricle, left
R1236138Hd-2	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Ventricle, right
R1236139Hd-2	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Small Intestine
R1236226Lcs-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Adipose
R1434003-50	Biochain	50 ug	EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

We current that the turbidity ensuing from LLPS could be utilized to detect the presence of specific nucleic acid sequences, through the use of the programmable CRISPR-nucleases Cas12a and Cas13a. Because of LLPS of polynucleotides and polycations causes choices to grow to be turbid, the detection of specific nucleic acid sequences may be seen with the naked eye. We moreover reveal that there is an optimum polynucleotide focus for detection. Lastly, we provide a theoretical prediction which hints in route of attainable enhancements of a LLPS primarily based detection assay. The deployment of LLPS enhances CRISPR-based molecular diagnostic functions and facilitates easy and low-cost nucleotide sequence detection.