Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5

The ApoE-/- mice had been fed a high-fat weight-reduction plan to assemble AS mouse fashions and the modeled mice had been dealt with with altered NORAD, miR-495-3p or KLF5. NORAD, miR-495-3p and KLF5 expression in mouse aorta tissues had been evaluated, and the levels of inflammatory components, oxidative stress components, endothelial carry out indices and blood lipid in mice had been all determined. The atherosclerotic plaque area, lipid deposition area, collagen fibers and CD68 expression in mouse aorta tissues had been assessed. The regulatory relation between NORAD and miR-495-3p, and the aim relation between miR-495-3p and KLF5 had been confirmed.  
NORAD and KLF5 had been elevated whereas miR-495-3p was decreased in atherosclerotic mouse aortas. Inhibited NORAD or elevated miR-495-3p suppressed irritation, oxidative stress, endothelial dysfunction, blood lipid stage, atherosclerotic plaque area, collagen fibers and CD68 expression in atherosclerotic mouse aortas. Outcomes of elevated miR-495-3p on atherosclerotic mice might very effectively be reversed by up-regulation of KLF5. NORAD served as a sponge of miR-495-3p and miR-495-3p instantly targeted KLF5.  Silenced NORAD elevated miR-495-3p to suppress atherosclerotic plaque formation by means of reducing KLF5. Findings in our evaluation may be helpful for exploring molecular mechanisms of AS. On this paper, we discovered that the main target of 5mdC, 5mrC, m6A from coronary coronary heart tissue significantly elevated at eight weeks after the surgical process.

Furthermore, UPLC-MS/MS helps us observe the identical change of the main target of those Three methylated biomarkers in peripheral blood after eight weeks. The consequence displays that the dynamic technique of those Three methylated biomarkers in peripheral blood is alleged to the content material materials of methylated biomarkers from the middle tissue. Based mostly totally on the scientific proof on the market, we proved that the methylation of genetic provides in peripheral blood is rather like myocardial infarction tissue. The relation between them signifies that peripheral blood might very effectively be a promising completely different to the middle tissue which monitor the extent of methylation and MI diagnosis-aided.

A Simple Screening Method for Superior RNADNA Hybrid Nanoshapes

The design of hybrid nucleic acid nanomaterials capitalizes on the partitioning of architectural and sensible roles between structurally numerous RNA modules and chemically robust DNA components. Selecting optimum combos of RNA and DNA developing blocks is the vital factor to preparing safe polygonal RNA-DNA hybrid nanoshapes. Proper right here, we outline a simple screening method by gel electrophoresis beneath native folding circumstances to find out combos of RNA and DNA modules that self-assemble to robust polygonal hybrid nanoshapes. As a proof of thought, we outline the preparation of RNA-DNA hybrid nanoshapes containing a set of varied RNA architectural joints, along with inside loop motifs and three-way junction (3WJ) folds.

For each hybrid nanoshape, we reveal the selection course of used to find out optimum DNA modules from a library of DNA connectors. The simple screening method outlined proper right here presents a fundamental robust methodology to find out and put collectively RNA-DNA hybrid nanoshapes from numerous libraries of discrete nucleic acid developing blocks. End resection in homologous recombination (HR) and HR-mediated restore of DNA double-strand breaks (DSBs) removes quite a lot of kilobases from 5′ strands of DSBs, nonetheless 3′ strands are exempted from degradation.

The mechanism by which the three’ overhangs are protected has not been determined. Proper right here, we established that the security of three’ overhangs is achieved by the use of the transient formation of RNA-DNA hybrids. The DNA strand throughout the hybrids is the three’ ssDNA overhang, whereas the RNA strand is newly synthesized. RNA polymerase III (RNAPIII) is accountable for synthesizing the RNA strand. Furthermore, RNAPIII is actively recruited to DSBs by the MRN superior. CtIP and MRN nuclease train is required for initiating the RNAPIII-mediated RNA synthesis at DSBs. A lowered stage of RNAPIII suppressed HR, and genetic loss > 30 bp elevated at DSBs. Thus, RNAPIII is a obligatory HR problem, and the RNA-DNA hybrid is a obligatory restore intermediate for shielding the three’ overhangs in DSB restore.

Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5

Sequence-independent RNA sensing and DNA specializing in by a break up space CRISPR-Cas12a gRNA change

CRISPR utilized sciences an increasing number of require spatiotemporal and dosage administration of nuclease train. One promising method contains linking nuclease train to a cell’s transcriptional state by engineering data RNAs (gRNAs) to carry out solely after complexing with a ‘set off’ RNA. Nonetheless, regular gRNA change designs do not allow neutral selection of set off and knowledge sequences, limiting gRNA change software program. Proper right here, we reveal the modular design of Cas12a gRNA switches that decouples selection of those sequences. The 5′ end of the Cas12a gRNA is fused to 2 distinct and non-overlapping domains: one base pairs with the gRNA repeat, blocking formation of a hairpin required for Cas12a recognition; the alternative hybridizes to the RNA set off, stimulating refolding of the gRNA repeat and subsequent gRNA-dependent Cas12a train.

Using a cell-free transcription-translation system and Escherichia coli, we current that designed gRNA switches can reply to fully completely different triggers and aim fully completely different DNA sequences. Modulating the scale and composition of the sensory space altered gRNA change effectivity. Lastly, gRNA switches might very effectively be designed to sense endogenous RNAs expressed solely beneath specific progress circumstances, rendering Cas12a specializing in train relying on cell metabolism and stress. Our design framework thus extra permits tethering of CRISPR actions to cell states.

Total RNA from Mouse Normal Tissue: Stomach

R1334248-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Stomach

R1236248Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Stomach

R1235248-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Rat Normal Tissue: Stomach

R1434248-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach

R1234248-50 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Monkey (Rhesus) Normal Tissue: Stomach

R1534248-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Monkey (Cynomolgus) Normal Tissue: Stomach

R1534248-Cy 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Congenital heart disease: Heart

R1236122Hd-3 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach: Cardia

R1234250-10 10 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach: Corpus

R1234251-10 10 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach: Fundus

R1234252-10 10 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Stomach: Pylorus

R1234253-10 10 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Mouse Normal Tissue: Stomach

P1334248 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Adult Normal Tissue 5 Donor Pool: Stomach

R1234248-P 50 ug
EUR 328
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Matching Pair - Total RNA from Human Primary and Metastatic Tumor Tissue: Stomach

R8235248-PM-10 2x10 ug
EUR 650
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Matching Pair - Total RNA from Human Primary Tumor and Normal Tissue: Stomach

R8235248-PP-10 2x10 ug
EUR 439
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL 96 assays
EUR 572

Total Protein from Human Tumor Tissue: Stomach

P1235248 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Diabetic Tissue: Stomach

P1236248Dia 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

RNA isolater Total RNA Extraction Reagent

R401-01 100 ml
EUR 133

Green RNA Lysis Kit 50

GREENE1-RNA 50pack
EUR 180
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RNA Lysis Kit 250

GREENE5-RNA 250pack
EUR 571
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Navy RNA Lysis Kit 50

NAVYE1-RNA 50pack
EUR 198
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RNA Lysis Kit 250

NAVYE5-RNA 250pack
EUR 651
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Pink RNA Lysis Kit 50

PINKE1-RNA 50pack
EUR 178
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RNA Lysis Kit 250

PINKE5-RNA 250pack
EUR 561
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 50

REDE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RNA Lysis Kit 250

REDE5-RNA 250pack
EUR 622
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 50

YELLOWE1-RNA 50pack
EUR 192
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RNA Lysis Kit 250

YELLOWE5-RNA 250pack
EUR 624
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Total RNA Extraction Kit

K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA Isolation Kit

P-9100 50 samples
EUR 347.25
Description: The best epigenetics products

Paraffin Tissue Section - Human Stomach Tumor: Adenocarcinoma, Well Differentiated

T2235248-3 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

5E GREEN RNA Lysis Kit 100

GREEN5E100-RNA 100pack
EUR 386
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 1000, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

5E GREEN RNA Lysis Kit 20

GREEN5E20-RNA 20pack
EUR 138
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 50

GREENR1-RNA 50pack
EUR 184
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

Green RINO RNA Lysis Kit 250

GREENR5-RNA 250pack
EUR 587
Description: Bead lysis kits for the homogenization of small, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with beads. RNase free.

5E Navy RNA Lysis Kit 100

NAVY5E100-RNA 100pack
EUR 453
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

5E Navy RNA Lysis Kit 20

NAVY5E20-RNA 20pack
EUR 153
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 50

NAVYR1-RNA 50pack
EUR 202
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

Navy RINO RNA Lysis Kit 250

NAVYR5-RNA 250pack
EUR 666
Description: Bead lysis kits for the homogenization of large, tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stainless steel beads. RNase free.

5E PINK RNA Lysis Kit 100

PINK5E100-RNA 100pack
EUR 380
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E PINK RNA Lysis Kit 20

PINK5E20-RNA 20pack
EUR 136
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 50

PINKR1-RNA 50pack
EUR 181
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Pink RINO RNA Lysis Kit 250

PINKR5-RNA 250pack
EUR 576
Description: Bead lysis kits for the homogenization of small, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 100

RED5E100-RNA 100pack
EUR 429
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 100, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

5E RED RNA Lysis Kit 20

RED5E20-RNA 20pack
EUR 148
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 20, 5mL Eppendorf Safe-Lock tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 50

REDR1-RNA 50pack
EUR 195
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Red RINO RNA Lysis Kit 250

REDR5-RNA 250pack
EUR 638
Description: Bead lysis kits for the homogenization of large, soft or medium-tough samples. Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 50

YELLOWR1-RNA 50pack
EUR 199
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 50, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

YELLOW RINO RNA Lysis Kit 250

YELLOWR5-RNA 250pack
EUR 656
Description: Bead lysis kits for the homogenization of small cell suspensions (ex - E. coli). Pack of 250, 1.5mL RINO screw-cap tubes prefilled with stabilized ceramic beads. RNase free.

Total Protein from Human Adult Normal Tissue: Stomach

P1234248 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Emphysema: Lung

P1236152Ld-3 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Human Total PSA (t-PSA) ELISA Kit

PRB-5049-TOTAL-5 5 x 96 assays
EUR 2283

Total RNA from Mouse Normal Tissue: Adipose

R1334003-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Bladder

R1334010-50 50 ug
EUR 303
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Brain

R1334035-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Colon

R1334090-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Heart

R1334122-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Kidney

R1334142-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Liver

R1334149-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Lung

R1334152-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Pancreas

R1334188-50 50 ug
EUR 341
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Placenta

R1334200-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Rectum

R1334206-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Spleen

R1334246-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Testis

R1334260-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA Isolation Kit II

FATRS--001 100 preps
EUR 308

Total RNA Isolation Kit II

FATRS--050 50 preps
EUR 216

Total RNA ExtractionMini KitAnimal Tissues

FYG301-50P 50 Preps Ask for price

Total RNA ExtractionMini KitAnimal Tissues

FYG302-100P 100 Preps Ask for price

Total RNA ExtractionMini KitAnimal Tissues

FYG302-300P 300 Preps Ask for price

Total RNA ExtractionMini KitPlant Tissues

FYG303-100P 100 Preps Ask for price

Total RNA ExtractionMini KitPlant Tissues

FYG303-300P 300 Preps Ask for price

Total RNA ExtractionMini KitPlant Tissues

FYG303-50P 50 Preps Ask for price

Total RNA Isolation Kit (Tissue)

P-9102 50 samples
EUR 347.25
Description: reagents widely cited

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 347.25
Description: kits suitable for this type of research

Total RNA from Lupus: Colon

R1236090Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart

R1236122Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Kidney

R1236142Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Liver

R1236149Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Lung

R1236152Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Pancreas

R1236188Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Spleen

R1236246Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Uterus

R1236274Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

AnaPrep Total RNA Extraction Kit

Z1322015 1 kit (48 extractions) Including all required plastic disposables
EUR 308
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.

Total Protein from Human Adult Normal Tissue: Stomach: Cardia

P1234250 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Corpus

P1234251 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Fundus

P1234252 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Pylorus

P1234253 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Congenital heart disease: Heart

P1236122Hd-3 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells

2-82088 1 Kit Ask for price

Total RNA from Mouse Normal Tissue: Whole Eye

R1334108-50 50 ug
EUR 296
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Skeletal Muscle

R1334171-50 50 ug
EUR 152
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Mouse Normal Tissue: Small Intestine

R1334226-50 50 ug
EUR 214
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Plant Total RNA Mini Kit (50prep)

FAPRK-001 50 preps
EUR 165

Plant Total RNA Mini Kit (100prep)

FAPRK-001-1 100 preps
EUR 216

Plant Total RNA Mini Kit (300prep)

FAPRK-001-2 300 preps
EUR 404

Plant Total RNA Maxi Kit (10prep)

FAPRK-002 10 preps
EUR 201

Plant Total RNA Maxi Kit (24prep)

FAPRK-002-1 24 preps
EUR 317

Tissue Total RNA Mini Kit (50prep)

FATRK-001 50 preps
EUR 165

Tissue Total RNA Mini Kit (100prep)

FATRK-001-1 100 preps
EUR 216

Tissue Total RNA Mini Kit (300prep)

FATRK-001-2 300 preps
EUR 404

Tissue Total RNA Maxi Kit (10prep)

FATRK-003 10 preps
EUR 201

Tissue Total RNA Maxi Kit (24prep)

FATRK-003-1 24 preps
EUR 317

Total RNA ExtractionMini KitBlood/Cultured Cell

FYG301-300P 300 Preps Ask for price

Broad Range Total RNA Isolation Kit

K1341050 1 kit
EUR 266
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Alzheimer's Disease: Brain

R1236035Alz-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Pons

R1236071Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Colon

R1236090Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Colon

R1236090Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Esophagus

R1236106Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Interventricular Septum

R1236130Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Kidney

R1236142Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Liver

R1236149Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Lung

R1236152Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Diaphragm

R1236169Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Pancreas

R1236188Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Pancreas

R1236188Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Rectum

R1236206Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Small Intestine

R1236226Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Spleen

R1236246Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Zenoquick Whole Blood Total RNA Kit

Z5010-050 50 preps
EUR 482

Zenoquick Whole Blood Total RNA Kit

Z5010-100 2x50 preps
EUR 806

Rapid Animal Total RNA Extraction Kit

AT4181 50Preps
EUR 88.06
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

Rapid Animal Total RNA Extraction Kit

AT4182 250preps
EUR 202.25
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

Mouse Stomach Tissue Preparation Buffer: Normal Stomach Mucosa Epithelial Cells

9-80202 1 x 100 ml Ask for price

Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells Growth Medium

9-32088 5 x 100 ml Ask for price

96-well Total RNA Kit (1 plates)

FATRE-96001 1 prep
EUR 194

96-well Total RNA Kit (2 plates)

FATRE-96002 2 preps
EUR 281

96-well Total RNA Kit (4 plates)

FATRE-96004 4 preps
EUR 448

Total RNA Plus Mini Kit (50 prep)

FATRK-P-050 50 preps
EUR 179

Total RNA Plus Mini Kit (100 prep)

FATRK-P-100 100 preps
EUR 242

FastPure Cell/Tissue Total RNA Isolation Kit

RC101 50 rxn
EUR 229

Total RNA from Human Tumor Tissue: Adrenal

R1235004-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bladder

R1235010-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bone

R1235023-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Breast

R1235086-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Colon

R1235090-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Esophagus

R1235106-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Kidney

R1235142-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Liver

R1235149-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Lung

R1235152-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Nose

R1235178-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Ovary

R1235183-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Parotid

R1235190-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Rectum

R1235206-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Melanoma

R1235218A-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Synoviosarcoma

R1235257-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thymus

R1235264-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thyroid

R1235265-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Tonsil

R1235268-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Uterus

R1235274-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Corpus Callosum

R1236045Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Frontal Lobe

R1236051Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Occipital Lobe

R1236062Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Parietal Lobe

R1236066Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Postcentral Gyrus

R1236072Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Precentral Gyrus

R1236073Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Temporal Lobe

R1236078Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Atrium, left

R1236126Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Atrium, right

R1236127Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Ventricle, left

R1236138Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Ventricle, right

R1236139Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Small Intestine

R1236226Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

We current that the turbidity ensuing from LLPS could be utilized to detect the presence of specific nucleic acid sequences, through the use of the programmable CRISPR-nucleases Cas12a and Cas13a. Because of LLPS of polynucleotides and polycations causes choices to grow to be turbid, the detection of specific nucleic acid sequences may be seen with the naked eye. We moreover reveal that there is an optimum polynucleotide focus for detection. Lastly, we provide a theoretical prediction which hints in route of attainable enhancements of a LLPS primarily based detection assay. The deployment of LLPS enhances CRISPR-based molecular diagnostic functions and facilitates easy and low-cost nucleotide sequence detection.