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Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5

Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5

By June 8, 2021 Antibodies, Biology Cells, Blogging, colorless, Containing, Culture Cells, Enzyme, Epiquik, ethidium, exosome, Exosomes, extract, genome, GMO, Goat, Green, Horse serum, Panel, Reagents, RNA 0 Comments
The ApoE-/- mice had been fed a high-fat weight-reduction plan to assemble AS mouse fashions and the modeled mice had been dealt with with altered NORAD, miR-495-3p or KLF5. NORAD, miR-495-3p and KLF5 expression in mouse aorta tissues had been evaluated, and the levels of inflammatory components, oxidative stress components, endothelial carry out indices and blood lipid in mice had been all determined. The atherosclerotic plaque area, lipid deposition area, collagen fibers and CD68 expression in mouse aorta tissues had been assessed. The regulatory relation between NORAD and miR-495-3p, and the aim relation between miR-495-3p and KLF5 had been confirmed.  
NORAD and KLF5 had been elevated whereas miR-495-3p was decreased in atherosclerotic mouse aortas. Inhibited NORAD or elevated miR-495-3p suppressed irritation, oxidative stress, endothelial dysfunction, blood lipid stage, atherosclerotic plaque area, collagen fibers and CD68 expression in atherosclerotic mouse aortas. Outcomes of elevated miR-495-3p on atherosclerotic mice might very effectively be reversed by up-regulation of KLF5. NORAD served as a sponge of miR-495-3p and miR-495-3p instantly targeted KLF5.  Silenced NORAD elevated miR-495-3p to suppress atherosclerotic plaque formation by means of reducing KLF5. Findings in our evaluation may be helpful for exploring molecular mechanisms of AS. On this paper, we discovered that the main target of 5mdC, 5mrC, m6A from coronary coronary heart tissue significantly elevated at eight weeks after the surgical process.

Furthermore, UPLC-MS/MS helps us observe the identical change of the main target of those Three methylated biomarkers in peripheral blood after eight weeks. The consequence displays that the dynamic technique of those Three methylated biomarkers in peripheral blood is alleged to the content material materials of methylated biomarkers from the middle tissue. Based mostly totally on the scientific proof on the market, we proved that the methylation of genetic provides in peripheral blood is rather like myocardial infarction tissue. The relation between them signifies that peripheral blood might very effectively be a promising completely different to the middle tissue which monitor the extent of methylation and MI diagnosis-aided.

A Simple Screening Method for Superior RNA–DNA Hybrid Nanoshapes

The design of hybrid nucleic acid nanomaterials capitalizes on the partitioning of architectural and sensible roles between structurally numerous RNA modules and chemically robust DNA components. Selecting optimum combos of RNA and DNA developing blocks is the vital factor to preparing safe polygonal RNA-DNA hybrid nanoshapes. Proper right here, we outline a simple screening method by gel electrophoresis beneath native folding circumstances to find out combos of RNA and DNA modules that self-assemble to robust polygonal hybrid nanoshapes. As a proof of thought, we outline the preparation of RNA-DNA hybrid nanoshapes containing a set of varied RNA architectural joints, along with inside loop motifs and three-way junction (3WJ) folds.

For each hybrid nanoshape, we reveal the selection course of used to find out optimum DNA modules from a library of DNA connectors. The simple screening method outlined proper right here presents a fundamental robust methodology to find out and put collectively RNA-DNA hybrid nanoshapes from numerous libraries of discrete nucleic acid developing blocks. End resection in homologous recombination (HR) and HR-mediated restore of DNA double-strand breaks (DSBs) removes quite a lot of kilobases from 5′ strands of DSBs, nonetheless 3′ strands are exempted from degradation.

The mechanism by which the three’ overhangs are protected has not been determined. Proper right here, we established that the security of three’ overhangs is achieved by the use of the transient formation of RNA-DNA hybrids. The DNA strand throughout the hybrids is the three’ ssDNA overhang, whereas the RNA strand is newly synthesized. RNA polymerase III (RNAPIII) is accountable for synthesizing the RNA strand. Furthermore, RNAPIII is actively recruited to DSBs by the MRN superior. CtIP and MRN nuclease train is required for initiating the RNAPIII-mediated RNA synthesis at DSBs. A lowered stage of RNAPIII suppressed HR, and genetic loss > 30 bp elevated at DSBs. Thus, RNAPIII is a obligatory HR problem, and the RNA-DNA hybrid is a obligatory restore intermediate for shielding the three’ overhangs in DSB restore.

Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5

Sequence-independent RNA sensing and DNA specializing in by a break up space CRISPR-Cas12a gRNA change

CRISPR utilized sciences an increasing number of require spatiotemporal and dosage administration of nuclease train. One promising method contains linking nuclease train to a cell’s transcriptional state by engineering data RNAs (gRNAs) to carry out solely after complexing with a ‘set off’ RNA. Nonetheless, regular gRNA change designs do not allow neutral selection of set off and knowledge sequences, limiting gRNA change software program. Proper right here, we reveal the modular design of Cas12a gRNA switches that decouples selection of those sequences. The 5′ end of the Cas12a gRNA is fused to 2 distinct and non-overlapping domains: one base pairs with the gRNA repeat, blocking formation of a hairpin required for Cas12a recognition; the alternative hybridizes to the RNA set off, stimulating refolding of the gRNA repeat and subsequent gRNA-dependent Cas12a train.

Using a cell-free transcription-translation system and Escherichia coli, we current that designed gRNA switches can reply to fully completely different triggers and aim fully completely different DNA sequences. Modulating the scale and composition of the sensory space altered gRNA change effectivity. Lastly, gRNA switches might very effectively be designed to sense endogenous RNAs expressed solely beneath specific progress circumstances, rendering Cas12a specializing in train relying on cell metabolism and stress. Our design framework thus extra permits tethering of CRISPR actions to cell states.

Total RNA from Mouse Normal Tissue: Stomach
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R8235248-PM-10 2x10 ug
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P1235248 1 mg
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YELLOWE1-RNA 50pack
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YELLOWE5-RNA 250pack
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Total RNA Extraction Kit
K2014005 1 kit
EUR 344
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Paraffin Tissue Section - Human Stomach Tumor: Adenocarcinoma, Well Differentiated
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5E GREEN RNA Lysis Kit 100
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5E GREEN RNA Lysis Kit 20
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Green RINO RNA Lysis Kit 50
GREENR1-RNA 50pack
EUR 184
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Green RINO RNA Lysis Kit 250
GREENR5-RNA 250pack
EUR 587
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5E Navy RNA Lysis Kit 100
NAVY5E100-RNA 100pack
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EUR 153
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Navy RINO RNA Lysis Kit 50
NAVYR1-RNA 50pack
EUR 202
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Navy RINO RNA Lysis Kit 250
NAVYR5-RNA 250pack
EUR 666
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5E PINK RNA Lysis Kit 100
PINK5E100-RNA 100pack
EUR 380
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5E PINK RNA Lysis Kit 20
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EUR 136
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Pink RINO RNA Lysis Kit 50
PINKR1-RNA 50pack
EUR 181
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PINKR5-RNA 250pack
EUR 576
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RED5E100-RNA 100pack
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RED5E20-RNA 20pack
EUR 148
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Red RINO RNA Lysis Kit 50
REDR1-RNA 50pack
EUR 195
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Red RINO RNA Lysis Kit 250
REDR5-RNA 250pack
EUR 638
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YELLOW RINO RNA Lysis Kit 50
YELLOWR1-RNA 50pack
EUR 199
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YELLOW RINO RNA Lysis Kit 250
YELLOWR5-RNA 250pack
EUR 656
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Total Protein from Human Adult Normal Tissue: Stomach
P1234248 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Emphysema: Lung
P1236152Ld-3 1 mg
EUR 461
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Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL-5 5 x 96 assays
EUR 2283

Total RNA from Mouse Normal Tissue: Adipose
R1334003-50 50 ug
EUR 214
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Total RNA from Mouse Normal Tissue: Bladder
R1334010-50 50 ug
EUR 303
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Total RNA from Mouse Normal Tissue: Brain
R1334035-50 50 ug
EUR 152
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Total RNA from Mouse Normal Tissue: Colon
R1334090-50 50 ug
EUR 214
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Total RNA from Mouse Normal Tissue: Heart
R1334122-50 50 ug
EUR 152
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Total RNA from Mouse Normal Tissue: Kidney
R1334142-50 50 ug
EUR 152
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Total RNA from Mouse Normal Tissue: Liver
R1334149-50 50 ug
EUR 152
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Total RNA from Mouse Normal Tissue: Lung
R1334152-50 50 ug
EUR 152
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Total RNA from Mouse Normal Tissue: Pancreas
R1334188-50 50 ug
EUR 341
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Total RNA from Mouse Normal Tissue: Placenta
R1334200-50 50 ug
EUR 214
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Total RNA from Mouse Normal Tissue: Rectum
R1334206-50 50 ug
EUR 214
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R1334246-50 50 ug
EUR 152
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EUR 152
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FATRS--001 100 preps
EUR 308

Total RNA Isolation Kit II
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EUR 216

Total RNA ExtractionMini KitAnimal Tissues
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Total RNA ExtractionMini KitPlant Tissues
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Total RNA Isolation Kit (Tissue)
P-9102 50 samples
EUR 347.25
Description: reagents widely cited

Total RNA Isolation Kit (Plant)
P-9104 50 samples
EUR 347.25
Description: kits suitable for this type of research

Total RNA from Lupus: Colon
R1236090Lup-50 50 ug
EUR 351
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Total RNA from hypertension: Heart
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EUR 351
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Total RNA from Lupus: Kidney
R1236142Lup-50 50 ug
EUR 351
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Total RNA from Lupus: Liver
R1236149Lup-50 50 ug
EUR 351
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Total RNA from Lupus: Lung
R1236152Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Pancreas
R1236188Lup-50 50 ug
EUR 351
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Total RNA from Lupus: Spleen
R1236246Lup-50 50 ug
EUR 351
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R1236274Lup-50 50 ug
EUR 351
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AnaPrep Total RNA Extraction Kit
Z1322015 1 kit (48 extractions) Including all required plastic disposables
EUR 308
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Total Protein from Human Adult Normal Tissue: Stomach: Cardia
P1234250 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Corpus
P1234251 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Fundus
P1234252 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total Protein from Human Adult Normal Tissue: Stomach: Pylorus
P1234253 1 mg
EUR 214
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Total Protein from Congenital heart disease: Heart
P1236122Hd-3 1 mg
EUR 461
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Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells
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Total RNA from Mouse Normal Tissue: Whole Eye
R1334108-50 50 ug
EUR 296
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R1334171-50 50 ug
EUR 152
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Total RNA from Mouse Normal Tissue: Small Intestine
R1334226-50 50 ug
EUR 214
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Plant Total RNA Mini Kit (50prep)
FAPRK-001 50 preps
EUR 165

Plant Total RNA Mini Kit (100prep)
FAPRK-001-1 100 preps
EUR 216

Plant Total RNA Mini Kit (300prep)
FAPRK-001-2 300 preps
EUR 404

Plant Total RNA Maxi Kit (10prep)
FAPRK-002 10 preps
EUR 201

Plant Total RNA Maxi Kit (24prep)
FAPRK-002-1 24 preps
EUR 317

Tissue Total RNA Mini Kit (50prep)
FATRK-001 50 preps
EUR 165

Tissue Total RNA Mini Kit (100prep)
FATRK-001-1 100 preps
EUR 216

Tissue Total RNA Mini Kit (300prep)
FATRK-001-2 300 preps
EUR 404

Tissue Total RNA Maxi Kit (10prep)
FATRK-003 10 preps
EUR 201

Tissue Total RNA Maxi Kit (24prep)
FATRK-003-1 24 preps
EUR 317

Total RNA ExtractionMini KitBlood/Cultured Cell
FYG301-300P 300 Preps Ask for price

Broad Range Total RNA Isolation Kit
K1341050 1 kit
EUR 266
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Total RNA from Alzheimer's Disease: Brain
R1236035Alz-50 50 ug
EUR 351
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Total RNA from Alzheimer's Disease: Pons
R1236071Alz-10 10 ug
EUR 351
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Total RNA from Diabetic Disease: Colon
R1236090Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Colon
R1236090Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Esophagus
R1236106Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Interventricular Septum
R1236130Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Kidney
R1236142Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Liver
R1236149Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Lung
R1236152Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Diaphragm
R1236169Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Diabetic Disease: Pancreas
R1236188Dia-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Pancreas
R1236188Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Rectum
R1236206Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Lupus: Small Intestine
R1236226Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Spleen
R1236246Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Zenoquick Whole Blood Total RNA Kit
Z5010-050 50 preps
EUR 482

Zenoquick Whole Blood Total RNA Kit
Z5010-100 2x50 preps
EUR 806

Rapid Animal Total RNA Extraction Kit
AT4181 50Preps
EUR 88.06
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

Rapid Animal Total RNA Extraction Kit
AT4182 250preps
EUR 202.25
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal

Mouse Stomach Tissue Preparation Buffer: Normal Stomach Mucosa Epithelial Cells
9-80202 1 x 100 ml Ask for price

Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells Growth Medium
9-32088 5 x 100 ml Ask for price

96-well Total RNA Kit (1 plates)
FATRE-96001 1 prep
EUR 194

96-well Total RNA Kit (2 plates)
FATRE-96002 2 preps
EUR 281

96-well Total RNA Kit (4 plates)
FATRE-96004 4 preps
EUR 448

Total RNA Plus Mini Kit (50 prep)
FATRK-P-050 50 preps
EUR 179

Total RNA Plus Mini Kit (100 prep)
FATRK-P-100 100 preps
EUR 242

FastPure Cell/Tissue Total RNA Isolation Kit
RC101 50 rxn
EUR 229

Total RNA from Human Tumor Tissue: Adrenal
R1235004-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bladder
R1235010-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Bone
R1235023-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Breast
R1235086-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Colon
R1235090-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Esophagus
R1235106-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Kidney
R1235142-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Liver
R1235149-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Lung
R1235152-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Nose
R1235178-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Ovary
R1235183-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Parotid
R1235190-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Rectum
R1235206-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Melanoma
R1235218A-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Synoviosarcoma
R1235257-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thymus
R1235264-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Thyroid
R1235265-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Tonsil
R1235268-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Tumor Tissue: Uterus
R1235274-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Corpus Callosum
R1236045Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Frontal Lobe
R1236051Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Occipital Lobe
R1236062Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Parietal Lobe
R1236066Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Postcentral Gyrus
R1236072Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Precentral Gyrus
R1236073Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Alzheimer's Disease: Temporal Lobe
R1236078Alz-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Atrium, left
R1236126Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Atrium, right
R1236127Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Ventricle, left
R1236138Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from hypertension: Heart Ventricle, right
R1236139Hd-2 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Liver Cirrhosis: Small Intestine
R1236226Lcs-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

We current that the turbidity ensuing from LLPS could be utilized to detect the presence of specific nucleic acid sequences, through the use of the programmable CRISPR-nucleases Cas12a and Cas13a. Because of LLPS of polynucleotides and polycations causes choices to grow to be turbid, the detection of specific nucleic acid sequences may be seen with the naked eye. We moreover reveal that there is an optimum polynucleotide focus for detection. Lastly, we provide a theoretical prediction which hints in route of attainable enhancements of a LLPS primarily based detection assay. The deployment of LLPS enhances CRISPR-based molecular diagnostic functions and facilitates easy and low-cost nucleotide sequence detection.

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