RNA and DNA Research
Abstract Five overlapping Epstein-Barr virus (EBV) recombinants type 1 DNA fragments constituting a complete replication- and transformation-competent genome was cloned into cosmids and transfected together into P3HR-1 cells, along with a plasmid encoding the immediate early activator Z of EBV replication. P3HR-1 cells harbour an EBV type 2 that cannot transform primary B cells due […]
Introduction Escherichia coli Recombinants is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well established and has become the most popular expression platform. For this reason, there are many molecular tools and protocols available for the high-level production of heterologous proteins, such as a […]
Description To comprehensively analyze bacterial gene function, it is important to simultaneously generate multiple genetic modifications within the target gene. However, current genetic engineering approaches, primarily using suicide vectors or λ-red homologous recombination-based systems, are tedious and technically difficult to perform. Here, we develop a flexible and easy method to simultaneously construct multiple modifications at […]