RNA and DNA Research
Abstract Five overlapping Epstein-Barr virus (EBV) recombinants type 1 DNA fragments constituting a complete replication- and transformation-competent genome was cloned into cosmids and transfected together into P3HR-1 cells, along with a plasmid encoding the immediate early activator Z of EBV replication. P3HR-1 cells harbour an EBV type 2 that cannot transform primary B cells due […]
Introduction Escherichia coli Recombinants is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well established and has become the most popular expression platform. For this reason, there are many molecular tools and protocols available for the high-level production of heterologous proteins, such as a […]
Description To comprehensively analyze bacterial gene function, it is important to simultaneously generate multiple genetic modifications within the target gene. However, current genetic engineering approaches, primarily using suicide vectors or λ-red homologous recombination-based systems, are tedious and technically difficult to perform. Here, we develop a flexible and easy method to simultaneously construct multiple modifications at […]
Abstract Hybridomas were produced that secrete monoclonal antibodies (MAbs) specific for all members of the genus Leptospira (clone LF9) and those that are specific only for the pathogenic species (clones LD5 and LE1). MAb LF9, which was immunoglobulin G1 (IgG1), reacted with a 38 kDa component of whole-cell lysates separated by sodium dodecyl sulfate-polyacrylamide gel […]
Description Invitrogen’s HiiScript II reverse transcriptase is a genetically modified MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate RNA degradation during first-strand cDNA synthesis, resulting in higher yields of full-length cDNA. HiiScript RTs are the […]
Overview of STI12 There are currently more than 100 known genotypes of the human papillomavirus (HPV) associated with a number of diseases that include many types of cancers, in particular cervical cancer. The types of HPV are often called high-risk and low-risk, based on whether they put a person at risk for cancer. Two types […]
Abstract The focus of this article is how interpretations of laboratory data can utilize individual and population reference intervals, while taking full advantage of routine test procedures along with some of the newer laboratory tests, which can complement the ones. existing tests in the diagnosis of diseases. Keywords: Biochemistry; endocrine disease; Hematology; New tests; Reference […]
Expected use The A + CheQ COVID-19 High-Speed RT-qPCR Detection Kit is an in vitro kit Diagnosis (IVD) Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) test for the qualitative detection of SARS-CoV-2 viral nucleic acids in human superior clinical specimens (such as nasopharyngeal and oropharyngeal specimens swab) that are suspected of COVID-19 by your healthcare provider. […]